ZFIN Image: Olena et al., 2015, Fig. S5

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Figure Caption

Fig. S5

Tg(Tp1:GFP) reporter reveals changes in Notch signaling upon perturbation of miR-216a and snx5. Embryos (Tg(Tp1:GFP)) were injected at the 1-cell stage with miR-216a, miR-216a^MOs, snx5^MOs, snx5, or dye control (DIC). Embryos were grown in egg water until 24 hpf, at which point they were photographed using a fluorescence dissecting scope. Top panel shows Tp1 reporter fluorescence in green superimposed onto images of fish; bottom panel shows Tp1 reporter fluorescence in white. We observed a decrease in reporter fluorescence in miR-216a and snx5^MOs injected embryos, suggestive of lower Notch activation, and an increase in reporter fluorescence in miR-261a^MOs and snx5 injected embryos, suggestive of higher Notch activation, as compared to DIC.

Acknowledgments

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Reprinted from Developmental Biology, 400(1), Olena, A.F., Rao, M.B., Thatcher, E.J., Wu, S.Y., Patton, J.G., miR-216a regulates snx5, a novel notch signaling pathway component, during zebrafish retinal development, 72-81, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.