Fig. S3

Fig. S3 Sox4 knockdown causes ectopic proliferation in the ganglion cell layer. (A-B) Clusters of PNCA- and PH3-positive cells were observed in the GCL of sox4 morphant retinas at 72 hpf (B, dashed line), whereas in control morphants proliferative cells were only observed in the CMZ. (C-D) Pax6a was expressed in the GCL and inner half of the INL in control and sox4 morphants at 72 hpf; however a dense clump of extra cells in the GCL of sox4 morphants was pax6a-negative (D, dashed line). (E-F) Immunostaining of control and sox4 morphant retinas with the ganglion cell marker Zn-8. Clusters of Zn-8-negative cells were present in the GCL of sox4 morphants (F, dashed line). D, dorsal; V, ventral; L, lens; CMZ, ciliary marginal zone; GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; ON, optic nerve.