ZFIN Image: Wen et al., 2015, Fig. 5

Image

Figure Caption

Fig. 5

Hh signaling is elevated in Sox4-deficent zebrafish. (A–D′) Representative eye and whole body images of vehicle-treated and cyclopamine-treated sox4 morphants at 24 and 48 hpf. (E) Quantification of the percentage of coloboma in vehicle- and cyclopamine-treated control and sox4 morphants (control morphants+vehicle: 1.14±0.96% coloboma, n=2/175; control morphants+cyclopamine: 0.49±0.79% coloboma, n=1/205; sox4 morphants+vehicle: 57.78±2.28% coloboma, n=104/180; sox4 morphants+cyclopamine: 15.87±2.20% coloboma, n=26/165; Fisherós exact test, P<0.0001). (F–I) Representative eye and body lateral images (F–G′) and transverse sections (H, I) of ptch2:EGFP(+/) transgenic embryos injected with control (F-F′, H) or sox4 MO (G-G′, I) at 12 hpf. (J) qPCR analysis of ptch2 expression in embryos injected with control or sox4 MO at 8, 10, and 12 hpf. Relative transcript abundance was normalized to level of atp5h (three biological replicates; Student′s t-test, P<0.01).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 399(1), Wen, W., Pillai-Kastoori, L., Wilson, S.G., Morris, A.C., Sox4 regulates choroid fissure closure by limiting hedgehog signaling during ocular morphogenesis, 139-53, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.