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Fig. 5
Migration Characteristics of Stable-Bleb Cells In Vitro
(A) Illustration of progenitor cells cultured under serum/LPA-free (I, II) or serum/LPA-containing (III, IV) conditions with (II, IV) or without (I, III) confinement.
(A′) Table summarizing cell migration characteristics for different culture conditions.
(B?D) N-Cadherin-GFP (B), Ezrin-YFP (C), and Integrinα5-Integrinβ1-BiFc-Venus (D) localization in polarized stable-bleb cells.
(E) Representative tracks of migrating stable-bleb cells.
(F) Mean-square displacement (MSD) analysis data (blue, n = 67) with fit to a persistent random walk model (gray) and compared to random (~t, orange) and directed migration (~t2, red). Black dashed line highlights random migration on timescales above the persistence time Pt ~10 min (persistence length H150 µm).
(F′) Sketch of MSD time point analysis. Arrows denote variations in the direction of movement affecting persistence of cell movement.
(G) Average instantaneous stable-bleb cell migration speeds in confinement between glass-agarose or glass-glass assays for varying substrate coatings as indicated.
(H?J) Localization of Paxillin-GFP for stable-bleb cells on BSA- (H), PEG- (I), and Fibronectin-coated (J) glass substrates in confinement.
(K) Paxillin-GFP localization for mesodermal progenitors cultured on 2D planar substrates coated with Fibronectin. n denotes number of cells. White dashed lines in (H)?(K) outline cell contact areas. All scale bars represent 10 µm.
See also Extended Experimental Procedures.
Reprinted from Cell, 160, Ruprecht, V., Wieser, S., Callan-Jones, A., Smutny, M., Morita, H., Sako, K., Barone, V., Ritsch-Marte, M., Sixt, M., Voituriez, R., Heisenberg, C.P., Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility, 673-685, Copyright (2015) with permission from Elsevier. Full text @ Cell