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Fig. 7

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ZDB-IMAGE-150406-6
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Figures for Tamplin et al., 2015
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Figure Caption

Fig. 7

Modulating HSPC Niche Colonization with Lycorine Has Long-Term Effects on the Stem Cell Pool

(A) Lycorine treatment dose-dependently increases the percentage of embryos with high cmyb/runx1 expression levels, as shown in Figure S6E (linear regression of Y [response] on X [dose] is significant: r2 = 0.146607, p = 6.46 × 109).

(B and C) Stage-matched frames from parallel time-lapse movies show more HSPCs (arrowheads) with (C) 25 µM lycorine treatment than (B) DMSO-treated controls (42 hpf, Runx:GFP;kdrl:RFP).

(D) HSPCs counted in each frame and then averaged each hour. Five movies imaged in parallel: n = 2 controls and n = 3 lycorine treated.

(E) HSPCs in the same embryos were scored for continuous hours tracked in CHT. Longer median time HSPCs spent in CHT of lycorine-treated embryos was significant (1.67 hr; Wilcoxon signed-rank test, p = 0.01; HSPC counts normalized because treatment group was n = 3 and control group was n = 2).

(F and G) Pools of Runx:GFP+ embryos treated with lycorine from 2–3 dpf and washed off had significantly increased HSPC at (F) 7 dpf (DMSO versus 75 µM, p = 0.0004; flow cytometry analysis of four independent pools per dose) and (G) 4 months (DMSO versus 75 µM, p = 0.006).

Error bars show mean ± SEM. See also Figure S6.

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Reprinted from Cell, 160, Tamplin, O.J., Durand, E.M., Carr, L.A., Childs, S.J., Hagedorn, E.J., Li, P., Yzaguirre, A.D., Speck, N.A., Zon, L.I., Hematopoietic Stem Cell Arrival Triggers Dynamic Remodeling of the Perivascular Niche, 241-252, Copyright (2015) with permission from Elsevier. Full text @ Cell