ZFIN Image: Kjaer-Sorensen et al., 2014, Fig. 6

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Figure Caption

Fig. 6

papp-a2 knockdown causes angiogenesis defects and modulates vascular Notch signaling activity. (A,B) Representative z-stack projections of fluorescence microscopy images of control and papp-a2-knockdown Tg(fli1a:eGFP) zebrafish embryos at 26 hpf (A) and 48 hpf (B). Arrows, ISVs extending across somites; asterisks, abnormal ISV branching. (C) Representative z-stack projections of fluorescence microscopy images of control and papp-a2-knockdown embryos at 48 hpf in Notch activity reporter zebrafish embryos [Tg(Tp1bglob:eGFP)^um13]. Knockdown was performed using 1.25ng ATG, 5.0ng e1i1, or 2ng i3e4 papp-a2-targeted morpholino per embryo. As a negative control, 5 ng cMO was injected. All pictures are lateral views, anterior to the left and dorsal to the top. (D) Number of Notch-signaling positive (the mean±s.d. is indicated) ISVs in the tails of 48 hpf control and papp-a2-knockdown embryos. *P<0.05, **P<0.01; n = 11 (cMO), 10 (e1i1), 8 (ATG), 8 (i3e4). (E) Total number of ISVs (the mean ± s.d. is indicated) in the tails of 48 hpf control and papp-a2 knockdown Tg(fli1a:eGFP) embryos. n = 6 per treatment.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.