ZFIN Image: Kok et al., 2015, Fig. 3

Image

Figure Caption

Fig. 3

Normal Vascular and Lymphatic Development in nrp1a and mmp2 Mutant Embryos

(A) Schematic of Nrp1a domain structure, indicating position of nrp1a^hu9963. CUB, CUB domain; FA58C, coagulation factor 5/8 C-terminal domain; MAM, MAM domain; and DUF3481, domain of unknown function.

(B and C) Confocal micrograph of embryos bearing Tg(kdrl:egfp)^s843 (green) and Tg(gata1a:dsRed)^sd2 (red) transgenes at 5 days postfertilization. (B) Wild-type: DA and ISV are indicated. (C) nrp1a^hu9963 mutant: ISV carrying red blood cells is indicated.

(D) Schematic of the Mmp2 and mmp2^hu10535 allele. SP, signal peptide; PG-binding, peptidoglycan-binding domain; ZnMc, zinc-dependent metalloprotease; and HX, hemopexin-like repeats.

(E and F) Confocal micrographs of embryos bearing Tg(flt4:mCitrine)^hu7135 (green) and Tg(flt1:tdTomato)^hu5333 (red) transgenes. (E) Wild-type: ISLV, intersegmental artery (ISA), DA, and TD are indicated. (F) mmp2^hu10535 mutant embryo.

Figure Data

Phenotype details

Acknowledgments

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Reprinted from Developmental Cell, 32(1), Kok, F.O., Shin, M., Ni, C., Gupta, A., Grosse, A.S., van Impel, A., Kirchmaier, B.C., Peterson-Maduro, J., Kourkoulis, G., Male, I., DeSantis, D.F., Sheppard-Tindell, S., Ebarasi, L., Betsholtz, C., Schulte-Merker, S., Wolfe, S.A., Lawson, N.D., Reverse Genetic Screening Reveals Poor Correlation between Morpholino-Induced and Mutant Phenotypes in Zebrafish, 97-108, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell