ZFIN Image: Zhao et al., 2014, Fig. S4

Image

Figure Caption

Fig. S4 Jak/Stat3 signaling mediates injury-dependent activation of the ascl1a promoter. Related to Figure 3. (A) GFP and BrdU immunofluorescence co-localize beneath the injury site in 6-ascl1a:gfp fish at 4 dpi. (B) A distal 1.5 kb ascl1a promoter fragment is necessary for injury-dependent transgene induction. (C, D) Consensus Stat3 sites located within this distal 1.5 kb region are necessary for injury-dependent transgene expression. BrdU+ immunofluorescence identifies the injury site and a normal regenerative response. The asterisks mark the injury sites. See Figure 3 for promoter schematic. Scale bar, 50 µm. INL, inner nuclear layer.

Acknowledgments

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