ZFIN Image: Zhao et al., 2014, Fig. 7

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Figure Caption

Fig. 7

Leptin Synergizes with IL-11 and CNTF to Stimulate Jak/Stat3 Signaling in MG-Derived Progenitors

(A) Intravitreal injection of Leptin/CNTF, or Leptin/IL-11 into the eye of gfap:stat3-gfp fish stimulated Stat3-GFP expression and BrdU incorporation throughout the uninjured retina’s INL, whereas Leptin, CNTF, or IL-11 alone had little effect. Note that GFP reports activated p-Stat3 expression (Figures 1 and S2). White dots indicate autofluorescence unique to the green channel (see Figure S7K). The arrows point to MG-derived progenitors in the top four panels.

(B) Quantification of Stat3-GFP⁺ cells in (A). p < 0.001 (combination versus individual), n = 4.

(C) Quantification of BrdU⁺ cells in (A). p < 0.001 (combination versus individual), n = 4.

(D) Western blot shows that retinal injury or intravitreal injection of cytokines into an uninjured eye increases p-Stat3 expression. GS serves as the loading control.

(E) Model showing that Leptin and IL-6 family cytokines synergize to stimulate MG reprogramming and retina regeneration via a Jak/Stat3 signaling pathway, which is essential for activating reprogramming genes such as hbegf and ascl1a.

Error bars, SD. Scale bars, 50 µm.

Figure Data

Expression / Labeling details

Acknowledgments

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