ZFIN Image: Shao et al., 2009, Fig. 2

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Figure Caption

Fig. 2

Specificity and effectiveness of dscr5 MOs. (A) Rescue of CE defects in dscr5 morphants by mut-dscr5 RNA. Summary of the phenotypes at 25 hpf with numbers at the top indicating total embryos scored from four independent experiments. (B) Western blot analysis of the effect of dscr5MO on the translation of dscr5MT and mut-dscr5MT RNAs. Endogenous β-Catenin was used as a loading control. (C-F) Rescue of CE defects in embryos injected with MO3 and cultured to 27 hpf. (C) CoMO-injected embryos. (D) MO3-injected embryos. (E) Embryos coinjected with MO3 and dscr5 RNA. (F) Summary of the rescue from three experiments, with the number of total embryos indicated at the top. (G) Schematic representation and RT-PCR analysis at 12.5 hpf of MO3-induced mis-splicing of dscr5 pre-mRNA.

Figure Data

Phenotype details

Acknowledgments

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