ZFIN Image: Zhang et al., 2015, Fig. 2

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Figure Caption

Fig. 2

The down-regulation of dnd results in the disrupted migration and reduced number of PGCs in the TG3 embryos.

The mRNA level of (a) dnd, (b) nanos1, (c) tdrd7 in the TG3 and the WT embryos at the 1-cell, 1k-cell, oblong, 50%-epiboly, and 3-somite embryonic stages, validated by quantitative Q-PCR. The 2^ΔCt values were plotted as the relative mRNA level normalized to odc1. Values are represented as the mean ± SEM. of five repeats. *P < 0.05, ** P < 0.01. (d) Vasa-positive PGCs were found close to the yolk syncytial layer (YSL) in the WT embryos. At 4.3 hours post-fertilization (hpf) some PGCs (indicated by arrows) were far from the YSL in the TG3 embryos. (e) The number of vasa-positive PGCs decreased in the TG3 embryos compared with the WT embryos at 20 hpf and 24 hpf in the dorsal view. (Scale bars: 100 µm).

Acknowledgments

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