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Fig. S5
Expression analysis of gpr22 in zebrafish development.(A-D′) WISH for gpr22. (A, B, C, D) Lateral view with anterior to the left. (B′) Dorsal view of the tail bud region. (C′) Anterior view. (D′) Dorsal view with anterior to the top. (A, B) From shield to bud stage, gpr22 is ubiquitously expressed. (B′) From bud stage onwards, the expression pattern becomes more and more restricted to the axial structures and the developing KV (arrow). (C-D′) At later stages, gpr22 is expressed in several brain regions, the heart and some ciliated sensory organs, like (D) the retina and (D, D′) the nasal sac. WISH = whole-mount in situ hybridization, KV = Kupffer’s vesicle, hpf = hours post-fertilization, r = retina, ns = nasal sac.