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Fig. S4
gpr22 overexpression results in defective LR patterning. (A) Ventral or (B, C) dorsal views with anterior to the top of embryos at (A, B) 4 dpf or (C) 20 somite stage. (A) Transgenic embryos expressing GFP under control of the cmlc2 promoter. gpr22 overexpression results in (from left to right) normal, absent, reversed or bicardial cardiac looping (not shown). (B) WISH for foxa3. The liver of water injected control embryos develops at the left side of the embryo. In contrast, the position of the liver in embryos injected with gpr22 RNA is randomized (straight line). (C) WISH for southpaw (spaw). The expression of the early LR marker southpaw is randomized in gpr22 injected embryos (straight line). V = ventricle, A = atrium, dpf = days post-fertilization, GFP = green fluorescence protein, cmlc2 = cardiac myosin light chain type 2, LR = left-right, WISH = whole mount in situ hybridization.