ZFIN Image: Verleyen et al., 2014, Fig. 1

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Figure Caption

Fig. 1

The downwards bending of the tail in gpr22 morphants is rescued by the co-injection of gpr22 mRNA.

(A–C) Lateral views with anterior to the left of zebrafish embryos at 24 hpf injected at one cell stage with (A) water, (B) 0.2 mM fluorescein tagged gpr22 MO, (C) 75 pg/nl gpr22 mRNA or (D) 0.2 mM fluorescein tagged gpr22 MO and 100 pg/nl gpr22 mRNA. (B, C) Deregulation of gpr22 results in a bending of the tail, a reduction in length of the anterior-posterior axis and heart edema (arrow). (E) Quantification of the tail phenotype. (D, E) The curly tail of embryos injected with fluorescein tagged gpr22 MO is rescued by the co-injection of gpr22 mRNA in a dose-dependent manner. MO = morpholino, hpf = hours post-fertilization, n = number of analyzed embryos, WT = wild type.

Figure Data

Phenotype details

Acknowledgments

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