header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-IMAGE-150318-8
Figures for Gerlach et al., 2014

Gene:

Antibodies:

Figure Caption/Comments:

Fig. 4 prkCI/ζ loss of function disrupts apical-basal protein localization and prevents proper polarity establishment, though delayed tubulogenesis eventually occurs. Protein localization during pronephros development between the 22 ss through 36 hpf stages revealed alterations in double prkCI/ζ morphant nephrons compared to wild-type embryo controls. (A) At the 22 ss, double prkCI/ζ morphants had disorganized actin and lack Prkc at the apical domain, while Na+/K+ ATPase was diffusely spread over the entire cell membrane in both wild-types and double morphants. (B) At 28 ss, the Na+/K+ ATPase was still diffusely spread over the cell membrane in the morphants, but in wild-types this protein was restricted to the basolateral cell surface in a mutually exclusive domain to that of prkCI/ζ and actin, which defined the apical domain. Double prkCI/ζ morphants continued to show disorganized actin and lack prkCI/ζ at the 28 ss. (C) At 36 hpf, wild-type control and double prkCI/ζ morphant tubules were surrounded by the ECM component laminin. prkCI/ζ morphant embryos had unrestricted Na+/K+ ATPase and a single lumen was present, albeit less pronounced than in wild-type controls. (A–C) Scale bars, 5 µm. (D) Summary of pronephros tubulogenesis. Renal progenitors emerge as groups of mesenchymal cells that undergo rearrangements into circumferential clusters that display differential distribution of apical membrane components prior to lumen formation, at which time mutually exclusive domains of apical and basolateral proteins are established. Deficiency of prkCI/ζ disrupts timely apical protein localization, delays lumen formation, and discrete apical and basolateral domains are not created.

Figure Data:
Acknowledgments:
ZFIN wishes to thank the journal for permission to reproduce figures from this article. Please note that this material may be protected by copyright.