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Fig. 6
Zebrafish Abcc4 has functions in transport of MCB out of LLC-PK1 cells.
(A) Western blot analysis of Flag-tagged Abcc4 or Abcc4-G1188D in stably transfected LLC-PK1 cells and the control cells (CTRL) transfected with empty vectors. The β-actin was used as a loading control. (B) Green signals under the confocal microscope indicate that foreign Abcc4 or Abcc4-G1188D molecules are localized on plasma membrane of LLC-PK1 cells. (C) Fluorescence intensities in LLC-PK1 cells expressing Abcc4 or Abcc4-G1188D and the control (CTRL) after treatment with MCB for 30 min at indicated concentrations. (D) Fluorescence intensities in LLC-PK1 cells expressing Abcc4 or Abcc4-G1188D and the control (CTRL) after treatment with 25 µM MCB for indicated time periods. (E) Fluorescence intensities in Abcc4-expressing LLC-PK1 cells after treatment with MK571 for indicated time periods. Cells were incubated in medium containing 25 µM MCB and different concentrations of MK571. Data are expressed as means ± standard deviations (n = 3). Significant differences are indicated by *p<0.05 and **p<0.01.