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Fig. S10 Analysis of mpp5a morphants and double WISH of ectopic gene expression in the pronephros. Brightfield images show live wild-type control and mpp5a morphant at 72 hpf (lateral view, anterior to the left). WISH panels show wt1a (purple) and cdh17 (red). Wild-type embryos have mutually exclusive domains of wt1a in podocytes and cdh17 in the tubule, respectively. The minor class of mpp5a morphants with ectopic pronephros gene expression of wt1a has these transcripts located in the cdh17+ tubule (black arrowheads denote areas of co-staining).
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Reprinted from Developmental Biology, 396(2), Gerlach, G.F., Wingert, R.A., Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta, 183-200, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.