|
Fig. S11 Effect of the sox21a knockdown in the expression of dkk1b in the pLL primordium. A) The expression of dkk1b was detected by in situ hybridization (red) in the different backgrounds: Control (Control), sox21a morphant (MOsox21a), sox21a and p53 double morphant (MOsox21a+MOp53) and sox21a morpholino co-injected with a sox21a mRNA insensible to the morpholino (MOsox21a+sox21a). Injections were performed in a cldnb:gfp transgenic line allowing to visualize the pLL by GFP expression (green; right panels). White dotted lines mark the borders of the pLL primordium. B) Graph representing the percentage of pLL primordia with wild type or reduced expression of dkk1b in Controls (Control; n=15), sox21a morpholino (MOsox21a; n=56), double sox21a and p53 morpholino (MOsox21a+MOp53; n=7) and sox21a morpholino co-injected with sox21a mRNA ( MOsox21a+sox21a; n=10) backgrounds.