Fig. 5

Fig. 5 mSlc35c1 and GDP-Fuc can suppress Wnt8a induced patterning defects. (A–D) in situ hybridization of bozozok expression in injected embryos at sphere stage (4hpf). (E) Quantification of bozozok angle (area outlined by dashed lines in A–D). (F) qPCR of bozozok, a direct Wnt target gene, in embryos injected with wnt8a alone or combined with GDP-Fuc, gmds MO injected or mSlc35c1 expressing embryos. bozozok expression was normalized to the expression of the house keeping gene eef1a1a. (G) Posteriorization caused by over-expression of wnt8a. All embryos shown are 18hpf. P1-P4 denote the degree of posteriorization, as determined by the marker gene pax2a-krox20-myoD expression patterns. The P1 class lacks the telencephalon as indicated by loss of anterior pax2a expression, (red arrow). P2 embryos lack regions anterior to the MHB domain of pax2a, (red arrowhead). P3 embryos lack MHB expression of pax2a. In the P4 class krox20 expression indicates loss of anterior hindbrain segments. (H) Quantification of each treatment. Note: ^** Tukey′s procedure after ANOVA p<0.01. Null hypothesis for Χ² between embryos injected with wnt8a and with wnt8a plus mSlc35c1 p=6.05E-08.