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Fig. 5 Phenotypic changes following tBHQ exposure. Zebrafish embryos were exposed to tBHQ (5 or 10 µM) or 0.1% DMSO from 32?47 hpf. A. At 52 hpf, randomly selected embryos were mounted in 3% methylcellulose and imaged as described in Materials and Methods. B. Embryos were subsequently maintained in 0.3× Danieau′s water (without tBHQ) at until 120 hpf, when they were imaged again in order to assess recovery of pigmentation.
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