ZFIN Image: Campbell et al., 2013, Fig. S5

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Figure Caption

Fig. S5 casp-3aMO1 and casp-9MO1 morphants do not have increased apoptosis, and RGC axons follow normal trajectories to the optic tectum. (A–F) Lateral views of TUNEL labeling of WT (A), coMO (B), HA14-1 (activates Caspase-3 by the inhibition of Bcl-2, positive control; C), control (vehicle, Milli-Q water; D), casp-3aMO1 (E), and casp-9MO1 (F) at 80 hpf. No increase in apoptosis is observed in the morphants. (G–J) Lateral views of WT (G), coMO (H), casp-3aMO1 (I), and casp-9MO1 (J) morphants at 80 hpf, illustrating the pathway taken by one or a few YFP-Rab3–labeled RGCs from the optic chiasm to the tectum. Those morphant RGC axons that reach the tectum follow a normal trajectory. n = 5 embryos analyzed per condition. Tec, tectum. Anterior is left, and dorsal is up. Bar, 100 μm.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Biol.