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Fig. 5 CtBP1 localizes to synaptic ribbons, but does not disrupt endogenous Ribeye.
Representative images of immunolabel in posterior lateral line NM3 hair cells of 5 dpf larvae. Scale bars: 3 μm (main panels), 1 μm (insets). (A) Ribeye b antibody labeling of synaptic ribbons (magenta) and MAGUK antibody labeling of postsynaptic densities (yellow) in a WT sibling larva. Anti-myc (cyan) immunolabel was performed as a negative control. (B–C) CtBP1-myc (cyan), Ribeye b (magenta), and MAGUK (yellow) immunolabel in two representative transgenic larvae. (B) CtBP1-myc (cyan) is strongly localized to the nucleus with weak synaptic localization. The red arrow indicates a synaptic ribbon containing CtBP1-myc. Note that Ribeye immunolabel intensity in all four synaptic ribbons appears comparable. (C) CtBP1-myc (cyan) is weakly localized to the nucleus with strong synaptic localization. Presynaptic Ribeye immunolabel intensity is not reduced compared to WT (A).