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Fig. S1 smp expression during embryonic development of zebrafish, Related to Figure 1. (A) Sequence comparison between human SMP/FAM53B and FAM53A and C, genes most closely related to Smp/Fam53b: sequence identities are 31% between B and A and 33% between B and C as shown by the conserved amino acids highlighted grey. The black lines indicate the two homology regions in the SMP/FAM53B protein described in this paper. (B) Immunohistochemistry staining for zebrafish Smp shows the presence of the maternal protein in the 8-cell embryo. (C) Immunohistochemistry for Smp at 16-cell stage also detects the presence of the protein. (D) Immunohistochemistry for Smp at 90% epiboly. Lettered boxes indicate location of panels E-G. (E-G) Enlarged images of anterior (E), ventral (F) and dorsal (G) sides of a stained zebrafish embryo at 90% epiboly shows broad expression of Smp protein primarily in the nuclei. Scale bars equal 50 μm (A-D) and 15 μm (E-G).