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Fig. 6 Midbrain DA neuron specification is independent of Smo and Hh activity. All are ventral views of anti-TH stained embryos at 48 hpf. Anterior is upwards. (A,B) Specification of midbrain DA neurons is normal in smo mutant embryos (B), but the number is only about half that in wild-type embryos (A). (C,D) Inhibition of Hh activity during gastrulation does not impair the specification of midbrain DA neurons in either wild-type (C) or mutant (D) embryos. Arrows indicate ventral midbrain DA neurons.
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