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Fig. 8 smo and regulation of cell proliferation and cell death. (A,B) Lateral view of embryos at 26 hpf showing a general increase of cell death in smo mutants. Embryos in A are stained live with Acridine Orange. Embryos in B are stained using the TUNEL protocol. (C,D) Decreased cell proliferation in smo mutants at 28 hpf. Embryos were labeled with BrdU and stained using an anti- BrdU antibody. (C) Representative cross sections of the cervical region of stained embryos that show fewer labeled cells as well as lower total cell numbers in the mutant. The border of the spinal cord is outlined. (D) Graph that summarizes the cell proliferation data in the cervical spinal cord of three wild-type and three mutant embryos. Mitotic index is the percentage of BrdU-labeled cells in the neural tube. Error bar shows s.d.