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Fig. 4
Expression of Rgs14a during migration phases impairs PGC motility. (A and C) Forced expression of rgs14a-nanos32UTR in PGCs during migratory stages results in inefficient colonization of the gonad region at 24 hpf. Ectopic cells exemplarily labeled with arrowheads in A, n = number of embryos analyzed. (B) PGCs expressing Rgs14a exhibit shorter migration tracks, stemming from impaired motility parameters (D) and longer immotile phases (E). The results presented in B, D, and E were derived from time-lapse movies spanning 70 min (Movies S3 and S4) captured in three independent experiments, with 21 control and 35 rgs14a expressing PGCs analyzed. (F and G) PGCs expressing Rgs14a display an apolar cell shape and extensive protrusion formation (protrusions indicated by white arrowheads) (Movies S5 and S6), n = number of cells analyzed. (H) The RGS domain of Rgs14a is essential for the induction of ectopic PGC migration when expressed in PGCs under the control of the nanos 32 UTR. n = number of embryos analyzed. A, C, F, G, and H were controlled by injection of cd14-nanos32UTR RNA. B, D, and E were controlled by injection of gfp-nanos32UTR RNA. *P < 0.05 and **P < 0.01 using Student t test. Error bars indicate SEM.