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Fig. 2
Retinoic acid and Trazodone promote beta-cell proliferation without inducing hyperglycemia.
(A) Confocal images of Tg(ins:mAG-zGeminin(1/100))s947 + beta-cells (green) stained for Insulin (blue). The animals were treated with 1 µM retinoic acid, 10 μM trazodone or 10 μM prednisolone in 1% DMSO (scale bar = 20 μm). (B) Dose–response curves for Tg(ins:mAG-zGeminin(1/100))s947 + beta-cells showing the relationship between the concentration of the compounds and the number of proliferating beta-cells in larvae treated with retinoic acid, trazodone, or prednisolone in 1% DMSO from 3 to 4 dpf. Error bars represent SEM. *P<0.05, and ***P<0.005 compared to vehicle-treated controls; n = 16–20 larvae for each group. (C) Absolute glucose values in zebrafish larvae treated with retinoic acid, trazodone, or prednisolone in 1% DMSO. Error bars represent SEM. *P<0.05, and ***P<0.005 compared to vehicle-treated controls; n = 16–20 larvae for each group. (D) Blood glucose concentration in adult zebrafish treated with 30 μM prednisolone for 24 h. Error bars represent SEM. ***P<0.005 compared to vehicle-treated controls; n = 10 fish for each group.