ZFIN Image: Hudish et al., 2013, Fig. S2

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Figure Caption

Fig. S2

Pard3-EGFP Overexpression Phenocopies miR-219 Loss of Function

Representative spinal cord transverse images of 3 dpf heat-shocked control and heat-shocked Tg(hsp70l:pard3-EGFP) larvae processed for ZO-1 immunohistochemistry with dorsal to the top. (A) Control non-transgenic larva. ZO-1 is restricted to the central canal. (B and C) Examples of heat-shocked transgenic larvae showing strong Pard3-EGFP expression outside the spinal cord (outlined) and co-localized with ZO-1 to an apparent primitive lumen extending into dorsal spinal cord. (D) Example of a heat-shocked larva with an apparent double lumen (ectopic class) with co-localized Pard3-EGFP and ZO-1. (E) Graph showing number of larvae having dorsal expansion or ectopic co-localization of Pard3-EGFP (n=12 each for control and experimental) ***P=0.0002, ****P<0.0001. Scale bar equals 10 μm.

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Acknowledgments

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Reprinted from Developmental Cell, 27(4), Hudish, L.I., Blasky, A.J., and Appel, B., miR-219 Regulates Neural Precursor Differentiation by Direct Inhibition of Apical Par Polarity Proteins, 387-398, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell