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Figure Caption
Fig. S1 Wnt signaling defects in dkk1+ embryos. (A–D) Whole mount in situ hybridization (ISH) for mycn (A, B) and axin2 (C, D) expression in dkk1+ embryos at 24 hpf (2 hphs), lateral views, anterior to the left. Arrowheads indicate expression at the mid-hindbrain boundary. (E) Quantitative, real-time PCR (pPCR) analysis for axin2 (red bars) and lef1 (orange bars) in dkk1+ embryos, normalized to nontransgenic, heat-shocked controls, with ef1alpha as an internal control. Abbreviations: e, eye.
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