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Fig. 5
Developmental dynamics of E2 responsive tissues in Tg(5xERE:GFP) transgenic fish.
Zebrafish larvae were treated with 1 μM E2 (in 0.1% DMSO) from 3 hpf and imaged at 1 dpf (A-C), 2 dpf (D-F), 3 dpf (G-I), 4 dpf (J-L), 5 dpf (M-R) and 6 dpf (S-U). Arrows (white) indicate the liver; arrowheads (red) indicate the pancreas. A, D, G, J, M, P and S, bright-field images; B, E, H, K, N, Q and T corresponding GFP fluorescence images; C, F, I, L, O, R and U, overlay of bright-field and GFP images. A-C and S-U, lateral view; D-O, dorsal view; P-R, ventral view; anterior to the left. Scale bars, 100 μm.
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