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Fig. 14 Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014, with the exception that the PCR annealing temperature was increased to 60oC. Primer sequences were: Forward: CCATCCACTCATCACGTGTC. Reverse: AATTAACCCTCACTAAAGGGACTTGCTTGTTAGGCCAGGAG. PCR product size: 873 bp.
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