Fig. S11

Fig. S11

Detection of mitotic cells in spns1 mutants in the presence or absence of p53. (A) Phosphorylated histone H3 (pH 3) staining in spns1-mutant animals with normal or mutant p53 backgrounds. The UV (18 mj/cm²) treatment was done at 68 hpf, followed by the phenotype observations at 72 hpf. Scale bar, 250 μm. (B) Quantification of the pH 3-positive cells [in 27.2±3.2×10⁴ μm areas; the trunk region starting from the rostral start point of the yolk extension (the distal end of yolk) through the end of the caudal fin] shown in (A). Quantification of data presented in panel A (n = 9) is shown in the right graph; the number (n) of animals is for each genotype. Three independent areas (periderm or basal epidermal cells in the trunk) were selected from individual animals. Reduction of the pH 3 level was statistically significant in spns1^hi891/hi891 (spns1^-/-) animals in the presence of p53, and a reduced tendency (with no statistical significance) was also observed in spns1 mutants. Error bars represent the mean ± S.D., **p<0.05; *p<0.01; ns, not significant.