ZFIN Image: Sasaki et al., 2014, Fig. S5

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Figure Caption

Fig. S5

Undetectable apoptosis in spns1 and/or beclin 1 morphants. (A) In spns1 and/or beclin 1 morphants stained with acridine orange (green) and LysoTracker (red) cellular characteristics were compared with UV-treated specimens by using confocal microscopy at high magnification (×600). Scale bar, 10 μm. Quantification of data presented in A (n = 6) is shown in the right graphs; the number (n) of animals is for each morphant and uninjected (Uninj.) animal with or without UV treatment. Three independent areas (periderm or basal epidermal cells above the eye) were selected from individual animals. (B) TUNEL assays demonstrate apoptosis induction in UV-treated zebrafish embryos, but not in spns1 and/or beclin 1 morphants. The UV (18 mj/cm²) treatment was done at 36 hpf, and phenotypes were observed at 48 hpf. Scale bar, 10 μm. Quantification of the fluorescence intensities is shown at the right-side graph. Quantification of data presented in B (n = 6) is shown in the right graphs; the number (n) of animals is for each morphant and uninjected (Uninj.) animal with or without UV treatment. Three independent areas (periderm or basal epidermal cells above the eye) were selected from individual animals. Error bars represent the mean ± S.D., *p<0.005.

Acknowledgments

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