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Fig. 3
Convergent silencing of the zebrafish One Eyed Pinhead (OEP) gene with the β-actin promoter driving sense transcription and an inverted CMV promoter driving antisense transcription. The OEP open reading frame was directly cloned between the two promoters. (B–G) Compared to uninjected control embryos (UICs), CT-OEP injected embryos phenocopied OEP mutants with curved body axis, cyclopia (black arrow in C), and reduced notochord (red arrows in F compared to G). In 12% of the CT-OEP embryos, we observed a more severe phenotype with complete loss of eye development (red asterisk in E). (H) Graph showing % transient transgenics showing WT, classic OEP, or more severe phenotypes. n = 167. (I). Silencing of OEP is Dicer dependent. Embryos were injected as indicated in the absence or presence of Dicer MO or a control mismatch morpholino. **p < 0.005.