Fig. S16
Effect of admp knockdown on Chd mRNA and protein distribution. (a) chd mRNA expression in uninjected or admp-MO-injected embryos at the shield stage as revealed by in situ hybridization. The ratio of embryos with expanded expression of chd was indicated. (b) Chd protein distribution in uninjected or admp-MO-injected embryos at the shield stage revealed by immunostaining with anti-Chordin antibody. The shown were confocal images in animal-pole view with dorsal to the right. (c) Comparison of relative Chd intensity. The ratio of Chd to DAPI signals in the ventralmost or dorsalmost area was calculated in a way similar to that described in Supplementary Fig. S5. The ratio in the ventralmost area in uninjected embryos was set to 1.0.