|ZFIN ID: ZDB-IMAGE-140910-17|
Fine mapping of the chd promoter. (a) Illustration of reporter constructs carrying different lengths of the chd promoter. (b) Relative luciferase activity (RLA) in HEK293 cells transfected with different constructs. The control was transfection with the empty vector (pGL3) plasmid DNA. RLA was averaged from three independent experiments with SD indicated. (c) GFP expression in the organizer of shield stage embryos injected with different reporter constructs. (d, e) GFP expression in shield stage embryos injected with Tg(-0.235chd:gfp) alone or together with smad1-MO, Smad5-MO or both. Embryos were divided into 4 classes based on GFP intensity (d) and the ratio of embryos in each class was shown in (e). (f) Relative luciferase activity in Hep3B cells transfected with -0.235chd:luc reporter. The reporter was co-transfected with human Smad1 or Smad5. The empty vector pGL3 served as a control. Note that Smad5 transfection caused a significant reduction (p<0.05) of the reporter, but Smad1 transfection only led to a statistically nonsignificant reduction.
|Acknowledgments:||ZFIN wishes to thank the journal for permission to reproduce figures from this article. Please note that this material may be protected by copyright.|