The gtshβ promoter-driven GFP is expressed in pronephric tubules.
(A–D) dWISH analyses of gfp (red), wt1a1 (purple) and cdh17 (purple) in Tg(gtshβ::GFP) embryos at the somitogenic stages from 10 hpf to 24 hpf. (E–H) dWISH analyses of gfp (red) and slc20a1a (purple) in Tg(gtshβ::GFP) embryos at the somitogenic stages from 18 hpf to 24 hpf. (I) Nuance multispectral imaging of gfp-slc20a1a-double-stained Tg(gtshβ::GFP) embryos at 24 hpf. The podocytes stained with wt1a1 are indicated by arrows. The blue or red lines are drawn according to the expression patterns of the kidney tubule markers.
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