ZFIN Image: Howarth et al., 2014, Fig. 4

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Figure Caption

Fig. 4

De novo lipogenesis is enhanced in vitro and in vivo by nAtf6 overexpression.

A: ¹⁴C-acetate is preferentially incorporated into lipids in 5 dpf nAtf6 TG larvae compared to WT. The percent of ¹⁴C in lipid fraction was divided by ¹⁴C measured in the unextracted lysate from whole fish. The median fold change is noted; n = number of samples of pooled larvae. B: Oil red O staining of HepG2 cells transfected with GFP or nATF6. Bar = 10 μm. C: Quantification of lipid droplet number and area (square pixels). Statistics: unpaired t-test. *, p<0.05; n indicates the number of cells quantified over 20 fields from 2 independent experiments. D: Incorporation of ¹⁴C-acetate into lipids by HepG2 cells transfected with GFP or nATF6 normalized to protein. The median fold change is noted; n = number of separate batches of cells analyzed in 2 independent experiments.

Acknowledgments

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