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Fig. 4
Increased lipid levels in the ext2-/-fish coincide with decreased bone formation. A, Oil red O stain (ORO) in fish at 6dpf; B, the levels of lipids represented as absorbance of bounded ORO in 6 days old proteoglycan mutants: ext2, slc35b2, gpc4 and uxs1 (for more description see Additional file 2); C, the level of transcripts in 5-days old fish determined by quantitative PCR and normalised to slc25a5 level. Primer sequences are given in Table 1; D, whole mount fabp11a mRNA in situ hybridisation showing prematurely developing adipocytes in the belly of the ext2-/- fish. Error bars represent means with SEM.
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