|
Fig. S4 Excessive nitrite exposure did not affect hemodynamics of zebrafish embryos. Embryos were exposed with 100 mg/l nitrite from 10 hpf. After nitrite exposure, embryos derived from Tg(gata1:DsRed) exhibited normal development of red blood cells at 24 (A–D), 36 (E–H) and 48 hpf (I–L), respectively. Consistently, klf2a did not change its expression pattern at 48 hpf after nitrite exposure (M–N). Similarly, embryos derived from Tg(flk1:GFP) displayed normal vessel development at 24 (O-P, O2-P2), 36 (Q-R, Q2-R2) and 48 hpf (S-T, S2-T2), respectively. Panels O2-T2 are the magnification of the region outlined by rectangle in Panel O-T, respectively, showing the normal development of vessels.