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Fig. 2 Metastasis of primary human lung tumor cells in zebrafish. Cells derived from short term culture of a primary human lung tumor with an undifferentiated phenotype (#8) show extensive spread throughout the body of the fish (a, left) and invasion in vitro (a, right). In contrast, cells from a well-differentiated adenocarcinoma of the lung (#9) show only minimal dissemination in the fish and limited invasion in vitro. From cohort studies (b), the number of fish showing metastasis is significantly greater in #8 (shown as z-stack images using 40 × magnification). This relationship is maintained in in vitro invasion assays (b, right).