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Fig. 2
Ablation experiments suggest a contribution of thalamic-epithalamic early projecting cluster (ThEPC) cells to the habenulae. (a) Habenular nuclei at 4 dpf. dHbl, lateral dorsal habenula (red); dHbm, medial dorsal habenula (blue); vHb, ventral habenula (green); P, pineal (yellow); pp, parapineal (dark yellow). (b-e) MIP, anterior to the top, dorsal views of the habenular nuclei of 4 dpf Et(-1.0otpa:mmGFP)hd1 embryos, after (b) left and (c) right dHb ablation at 2 dpf. Asterisks mark the ablated side and white arrowheads highlight neurons located in the lateral habenular domain. The nuclei (in blue) are labeled with Sytox Orange. (d,e) Habenula formation at 4 dpf in (d) not ablated and in (e) ThEPC ablated Et(1.0otpa:mmGFP)hd1 embryos. Asterisk marks the ablated side. Same sized ellipses highlight the habenula size differences. The gamma was adjusted to a value of 0.80. lHb, left habenula; rHb, right habenula; ThEPC, thalamic-epithalamic early projecting cluster.