ZFIN Image: Park et al., 2013, Fig. 1

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Figure Caption

Fig. 1

Heterospecific recombination of rox and lox sites by Dre and Cre in zebrafish embryos.

(A, B) Schematic of ubb-Dre (A) and ubb-Cre (B) driver lines and corresponding Rox-Nuc-mCherrys-Rox and Lox-Nuc-mCherry-Lox reporters. Additional cryaa:Venus cassette facilitates identification of transgene-expressing embryos. Open triangles indicate Tol2 arms. (C) Images from double transgenic embryos produced by indicated crosses of ubb-Dre and ubb-Cre driver lines with either Rox-Nuc-mCherry-Rox-eGFP or Lox-Nuc-mCherry-Lox-eGFP reporter lines. Activation of eGFP confirms Dre-specific recombination of Rox-Nuc-mCherry-Rox reporter and Cre-specific recombination of Lox-Nuc-mCherry-Lox reporter. Scale bar: 200 μm.

Acknowledgments

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