ZFIN Image: George et al., 2014, Fig. 5

Image

Figure Caption

Fig. 5

SynJ1 is required for Golgi maintenance but not development.

The transgenic fish line Tg(TαCP:ER-GFP) was used to mark the ER. Retinal slices from 5 dpf WT (A) and nrc^a14 (B) larvae showed that the overall ER morphology was unaltered in the absence of SynJ1. Confocal images of Tg(crx:Man2a-GFP) WT (C, E, G) or nrc^a14 (D, F, H) larvae retina at 3–5 dpf showed that the Golgi is normal during photoreceptor development, but develops abnormalities disordered after cones become functional. No apparent abnormalities were seen in Golgi morphology of nrc^a14 compared to WT photoreceptors at 3 dpf (compare C and D). At 4 dpf, some mild morphology changes appeared in nrc^a14 Golgi (F, arrowhead). At 5 dpf, fragmented Golgi were visible in nrc^a14 photoreceptors (H, arrowheads). Man2a-GFP signal is shown in yellow, membranes of 3 dpf larvae were stained with BODIPY-TR and are shown in blue, and nuclei were stained with Hoechst and are shown in blue for 4 dpf and 5 dpf images. Syn = photoreceptor synapses, IS = inner segment. Scale bar = 2 μm in all images.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One