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Fig. 1
Standardized orientation of zebrafish embryos.
(A,B) Photographs of the brass tool for the simultaneous generation of agarose grooves within 96 well microtiter plates: (A) top view and (B) tilted view. For dimensions of the plate see Materials and Methods section. (C) Schematic depiction of a single well with a ventrally oriented embryo within an agarose cavity. Drawing is not to scale. (D) Illustrative example of aligned and oriented embryos. Shown are dorsal views of 48 hpf embryos acquired using a 2.5x objective on an inverted wide field screening microscope.
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