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Fig. 4
Puma is required for Bad-mediated radiosensitization.
(A) Shown are lateral views of representative tails from 27-hpf embryos exposed to 8 Gy IR after injection at the one-cell stage with 50 pg of mRNA encoding mcherry (cntl) or hBAD in addition to either 100 nmol of puma MO or mismatch (mm) control MO. Analysis of Caspase 3 activity shows that hBAD-mediated radiosensitivity (arrowheads) is dependent on puma expression. (B) Fluorescence intensity was measured in the spinal cords of at least 10 embryos from each group in (A). Data represent one experiment, but the experiment was independently performed three times with similar results.