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Fig. 1
Mst2 is essential for early zebrafish embryogenesis.
(A) Early developmental abnormalities of mst2 morphants. Control or mst2 morpholino (MO) at the indicated dose was injected into zebrafish embryos and phenotypes were analyzed at 52 hpf. Embryos were classified into five color categories on the basis of their phenotypes: blue, normal embryos; green, short body length (SL); orange, abnormal eye pigmentation (AP) accompanied by SL; red, abnormal eye morphology (AM) plus AP plus SL; and brown, dead or malformed embryos. Results are presented as the percentage of the total number of embryos examined (N). (B) Representative control and mst2 morphants at 52 hpf. Embryos were injected with control MO (13.3 ng) or mst2 MO (13.3 ng). Top panels, lateral views of whole embryos. Middle panels, higher magnification images of the head regions of the embryos in the top panels. Bottom panels, dorsal views of the head regions of the embryos in the top panels. (The head is at the top of each panel.) White arrowhead, representative area of AM.