Fig. 4
vegfd genetically interacts with ccbe1 and can compensate for loss of vegfc. (A-F) Confocal images of the trunk blood vasculature in 30hpf kdrl:egfp embryos injected with control MO (A-C), ccbe1 MO (D-F), 400pg vegfc mRNA (B,E) and 200pg vegfd mRNA (C,F). Injection of either vegfc or vegfd mRNA induced misguidance of the intersegmental blood vessels along the horizontal myoseptum (arrows). (G) The percentage of embryos with branched ISVs; numbers in brackets denote number of embryos scored. Silencing ccbe1 reduces the number of embryos with branched ISVs induced by injection of either vegfc or vegfd mRNA. (H-J) Confocal images of the trunk lymphatics in 5dpf lyve1:egfp embryos injected with vegfc MO-1+dsred mRNA (H), vegfc MO-1+400pg vegfc mRNA (I) and vegfc MO-1+200pg vegfd mRNA (J). Injection of either vegfc or vegfd mRNA can rescue the formation of the TD (asterisks) in vegfc morphant embryos. (K) Quantitation of TD formation at 5dpf. (L) Quantitation of TD formation at 5dpf in F1 progeny from an incross of vegfchu5055 heterozygous carriers. ***P<0.001, *P<0.05 by a Mann–Whitney test; error bars indicate s.d. Scale bars: 50μm.