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Fig. S1
Eye size in various compound mutants shows no obvious change in severity of the microphthalmia phenotype (compare to Figure 1C). A. Eye diameter along the anterior-posterior axis (orange line) was measured at 6dpf and normalized to body length (not including tail fin) (yellow line). Both normophthalmic and microphthalmic larvae are shown. B. Ratios of eye length to body length among the progeny of an in-cross of tbx2b+/lor fish show no obvious difference from wild type fish, (n = 220). C. The same ratios among the progeny of an in-cross of [gdf6a+/s327;tbx2b+/fby] fish show the expected Mendelian abundance of ~25% microphthlamic fish (see also Fig 3B). The normophthalmic fish have eye sizes distributed in a normal fashion (Shapiro-Wilk Normality test, p>0.05). Among the microphthalmic progeny, there is also a normal distribution of eye size (Shapiro-Wilk test, p>0.05) (n = 194).