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Fig. S7
Pharmacological inhibition of Duox1 inhibits H2O2 production after wounding. Zebrafish one-cell wild-type embryos were treated at 72 hpf by immersion in 100 μM DPI or vehicle alone (DMSO) in the presence of 50 μM acetyl-pentafluorobenzene sulphonyl fluorescein, and tailfins were then transected. Representative images of the formation of the H2O2 gradient at 1 h postwounding. Note that DPI treatment completely inhibits H2O2 formation at the wound. Scale bars, 100 μm.
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